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Chrysin protects cardiac H9c2 cells against H2O2-induced endoplasmic reticulum stress by up-regulating the Nrf2/PERK

Subramani Yuvaraj1, Arumugam Kalaiselvi Ajeeth1, Shanavas Syed Mohamed Puhari1

  • 1Molecular Cardiology Unit, Department of Biochemistry, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India.

Molecular and Cellular Biochemistry
|August 9, 2022
PubMed

Insights

Chrysin (Chy) protects heart cells from oxidative and ER stress by activating Nrf2 signaling, reducing apoptosis, and offering a potential therapy for cardiovascular diseases (CVDs). This natural flavonoid demonstrates cardioprotective effects through molecular mechanisms.

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Cellular Stress Response

Background:

  • Oxidative and endoplasmic reticulum (ER) stress are key contributors to cardiac apoptosis in cardiovascular diseases (CVDs).
  • Chrysin (Chy), a natural flavonoid, exhibits antioxidant and anti-apoptotic properties, but its precise role in CVDs and molecular mechanisms remain largely unelucidated.
  • Understanding Chy's interaction with ER stress pathways is crucial for exploring its therapeutic potential in CVDs.

Purpose of the Study:

  • To investigate the cardioprotective mechanisms of Chrysin (Chy) against oxidative and ER stress in H9c2 cardiomyoblast cells.
  • To elucidate the role of Nrf2 signaling in mediating the effects of Chy on ER stress reduction.
  • To explore the interaction of ER stress response proteins with Chy using computational and molecular approaches.

Main Methods:

  • H9c2 cells were pre-treated with Chrysin (Chy) and exposed to hydrogen peroxide (H2O2) to induce oxidative and ER stress.
  • Nrf2 signaling was investigated by transfecting cells with Nrf2 siRNA, followed by Chy treatment and H2O2 exposure.
  • Gene expression analysis (CHOP, GRP78, PERK, AFT6, PI3K, NF-κB, caspase-3), fluorescence staining for ROS, ER stress, apoptosis, and mitochondrial membrane potential (MMP) were performed.

Main Results:

  • Chrysin (Chy) pre-treatment significantly reduced H2O2-induced intracellular ROS generation, ER stress markers (CHOP, GRP78), and apoptosis.
  • Chy upregulated Nrf2-regulated gene expression and increased PERK and AFT6 expression, while downregulating PI3K, NF-κB, and caspase-3.
  • The protective effects of Chy were abrogated upon Nrf2 silencing, confirming its critical role in mediating Chy's cardioprotection.

Conclusions:

  • Chrysin (Chy) exerts significant cardioprotective effects by attenuating oxidative and ER stress-induced apoptosis in cardiomyoblast cells.
  • The mechanism involves the activation of Nrf2 signaling pathway, leading to reduced ER stress and cellular damage.
  • Enhancing Nrf2 signaling through Chy supplementation represents a promising therapeutic strategy for managing cardiovascular diseases (CVDs) associated with oxidative and ER stress.

Keywords:
ApoptosisChrysinEndoplasmic reticulum stressInflammationOxidative stress

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