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PTEN Immunohistochemistry
Simone Antunes Terra1, Pedro Luiz Toledo de Arruda Lourenção2,3, Maria Aparecida Marchesan Rodrigues
1From the Departments of Pathology (Terra, Rodrigues).
Archives of Pathology & Laboratory Medicine
|August 9, 2022
Summary
PTEN immunohistochemistry shows reduced expression in intestinal neuronal dysplasia type B (IND B), aiding diagnosis. This finding suggests neuromuscular junction involvement in IND B pathogenesis.
Area of Science:
- Gastroenterology
- Pathology
- Pediatric Surgery
Background:
- Intestinal neuronal dysplasia type B (IND B) is a complex enteric nervous system disorder causing infantile constipation.
- Current diagnostic criteria for IND B are debated, impacting clinical practice.
Purpose of the Study:
- To evaluate the utility of PTEN immunohistochemistry for diagnosing IND B.
- To compare PTEN expression in IND B cases with controls and Hirschsprung disease (HD).
Main Methods:
- PTEN immunohistochemical analysis of colorectal samples from 29 IND B cases, 4 controls, and 6 HD cases.
- Scoring of PTEN immunoexpression in glial cells and neural fibrils of the submucosal and myenteric plexuses and muscularis propria.
Main Results:
- Reduced or absent PTEN expression was found in submucosal and myenteric nerve plexuses in most IND B cases.
- Lack of PTEN expression in muscularis propria neural fibrils occurred in 72% of IND B cases.
- PTEN expression was positive in control and HD groups' neural structures.
Conclusions:
- PTEN immunohistochemistry is a valuable tool for diagnosing IND B.
- Reduced PTEN expression in the muscularis propria suggests neuromuscular junction involvement in IND B pathogenesis.
