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Updated: Sep 2, 2025
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
Comparison of simulated candidemia detection during prophylactic antifungal therapy
Yong Kwan Lim1, Oh Joo Kweon1, Hye Ryoun Kim1
1Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Background:
We investigated the neutralization performance of various automated blood culture systems for antifungal agents with regard to the most commonly isolated Candida species.
Methods:
In this study, we evaluated the time to detection (TTD) of simulated candidemia for 6 Candida spp. (C. albicans, C. auris, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis) in 3 automated blood culture systems (BACTEC™ FX, BACT/ALERT® 3D, and BACT/ALERT® VIRTUO®), with or without trough and peak levels of eight antifungal agents (amphotericin B, anidulafungin, caspofungin, fluconazole, itraconazole, micafungin, posaconazole, and voriconazole).
Results:
Caspofungin and micafungin significantly prolonged the TTDs for most of the tested strains in the 3 blood culture instruments, especially at peak concentrations.
Conclusion:
Peak concentrations of caspofungin and micafungin influence the performance of blood culture detection systems. Therefore, one should be careful about the possibility of prolonged TTDs for candidemia when using the abovementioned antifungal agents.
