Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

173
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
173
Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

34
Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
34

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond a Culture Plate.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Carbapenem resistance and associated resistance genes in enterobacteriaceae and non-enterobacteriaceae in India: A systematic review and meta-analysis.

The Indian journal of medical research·2026
Same author

Breathless. Tuberculosis, inequality and care in rural India.

The National medical journal of India·2026
Same author

Authors' response.

The Indian journal of medical research·2025
Same author

A hospital-based epidemiological study of the genes coding for carbapenemase production among carbapenem resistant Acinetobacter isolates in India.

Indian journal of medical microbiology·2025
Same author

Multicentric validation of the PathoDetect™ MTB RIF & INH assay for simultaneous detection of Mycobacterium tuberculosis, & drug resistance to rifampicin & isoniazid in presumptive pulmonary tuberculosis & drug-resistant TB patients.

The Indian journal of medical research·2025

Related Experiment Video

Updated: Sep 2, 2025

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

11.0K

NDM-beta-lactamase-1: Where do we stand?

Protiti Bose1, Aseem Rangnekar1, Prabha Desikan1

  • 1Department of Microbiology, Bhopal Memorial Hospital & Research Centre, Bhopal, Madhya Pradesh, India.

The Indian Journal of Medical Research
|August 10, 2022
PubMed
Summary

Multidrug-resistant Gram-negative bacilli, especially those with the New Delhi metallo-beta-lactamase-1 (NDM-1) gene, pose a significant threat. A rapid, cost-effective molecular assay is urgently needed for early detection and control.

Keywords:
CarbapenemaseGram-negative bacilliNDM-β-1enterobacteriaceaesusceptibility

More Related Videos

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K
Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

702

Related Experiment Videos

Last Updated: Sep 2, 2025

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

11.0K
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K
Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

702

Area of Science:

  • Microbiology
  • Molecular Epidemiology
  • Infectious Diseases

Background:

  • Multidrug-resistant Gram-negative bacilli (GNB), particularly carbapenem-resistant strains encoding the New Delhi metallo-beta-lactamase-1 (NDM-1) gene, are a growing concern in healthcare and community settings.
  • These highly resistant bacteria spread rapidly, driven by factors like antibiotic overuse and poor hygiene, posing a significant public health challenge, especially in India.
  • Existing molecular methods for detecting NDM-1 variants are often complex and not suitable for widespread field use.

Purpose of the Study:

  • To highlight the urgent need for a rapid, cost-effective molecular diagnostic tool for detecting NDM-1 and its variants.
  • To emphasize the importance of molecular epidemiology in understanding the spread of NDM-1 harboring strains.
  • To advocate for the development and implementation of field-deployable assays for early detection and containment.

Main Methods:

  • Review of current literature on multidrug-resistant Gram-negative bacilli and NDM-1 epidemiology.
  • Discussion of the limitations of existing diagnostic methods for carbapenemase detection.
  • Exploration of the potential of loop-mediated isothermal amplification (LAMP) as a rapid field detection method.

Main Results:

  • NDM-1 variants are rapidly emerging and spreading globally, necessitating enhanced surveillance.
  • Current diagnostic approaches are insufficient for timely detection in resource-limited settings.
  • Loop-mediated isothermal amplification (LAMP) shows promise as a sensitive and specific method for detecting MBL-producing bacteria.

Conclusions:

  • A rapid, accessible molecular diagnostic assay, such as LAMP, is crucial for combating the spread of NDM-1.
  • Effective strategies require integrated approaches including molecular surveillance and improved infection control measures.
  • Early detection at the grassroots level is essential to curb the antimicrobial resistance crisis.