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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

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Updated: Sep 2, 2025

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

microRNA-29a Regulates ADAM12 Through Direct Interaction With ADAM12 mRNA and Modulates Postischemic Perfusion

Victor Lamin¹, Joseph Verry¹, Olumayowa S Dokun¹

¹Division of Endocrinology and Metabolism, Carver College of Medicine University of Iowa Iowa City IA.

Journal of the American Heart Association

|August 10, 2022

Summary

This summary is machine-generated.

MicroRNA-29a (miR-29a) suppresses ADAM12 expression by binding to its mRNA, impairing blood flow recovery in peripheral artery disease. Modulating miR-29a offers a potential therapeutic target for vascular pathology.

Keywords:

ADAM12 angiogenesis hind limb ischemia miR‐29a and peripheral artery disease

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Last Updated: Sep 2, 2025

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

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Published on: June 11, 2020

A Modified Surgical Model of Hind Limb Ischemia in ApoE-/- Mice using a Miniature Incision

A Modified Surgical Model of Hind Limb Ischemia in ApoE-/- Mice using a Miniature Incision

Published on: May 13, 2021

Area of Science:

Vascular Biology
Molecular Medicine
Gene Regulation

Background:

Peripheral artery disease (PAD) involves blocked leg vessels, impacting blood flow.
Angiogenesis aids blood flow restoration in PAD.
ADAM12 is a key genetic modifier of perfusion recovery in PAD models.

Purpose of the Study:

Investigate the regulatory role of microRNA-29a (miR-29a) on ADAM12 expression in ischemia.
Determine the physiological effects of miR-29a modulation on angiogenesis and perfusion recovery in a nondiabetic setting.

Main Methods:

Overexpression and inhibition of miR-29a in ischemic mouse hind limbs.
Quantification of perfusion recovery, ADAM12 expression, angiogenesis, and muscle regeneration.
RNA immunoprecipitation assays to study miR-29a and ADAM12 mRNA interaction.

Main Results:

miR-29a overexpression decreased ADAM12 mRNA, increased muscle injury, and impaired angiogenesis and perfusion recovery.
miR-29a directly binds to ADAM12 mRNA, displacing it from the AGO-2 complex.
No significant effects on skeletal muscle regeneration or myofiber size were observed.

Conclusions:

miR-29a directly suppresses ADAM12 expression, leading to impaired vascular function and perfusion.
Elevated miR-29a levels may contribute to vascular pathology in conditions like diabetes and aging.
Modulation of miR-29a presents a potential therapeutic strategy for PAD.