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Preliminary study of hypoxia markers in diffuse large B-cell lymphoma
Eko A Pangarsa1, Probo Wuryantoro2, Ridho M Naibaho3
1Division of Hematology/Medical Oncology, Department of Internal Medicine, Medical Faculty of Diponegoro University, Dr Kariadi Hospital, Semarang, Central Java 50244, Indonesia.
Molecular and Clinical Oncology
|August 11, 2022
Summary
Hypoxia markers, hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor A (VEGF-A), are overexpressed in most diffuse large B-cell lymphoma (DLBCL) tumors. These findings suggest hypoxia may play a role in DLBCL pathogenesis.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Hypoxia is linked to solid tumors, but its role in diffuse large B-cell lymphoma (DLBCL) is unclear.
- Investigating hypoxia markers can reveal insights into DLBCL tumor biology.
Purpose of the Study:
- To assess the expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor A (VEGF-A) in DLBCL.
- To analyze the correlation of HIF-1α and VEGF-A with clinical and pathological features in DLBCL patients.
Main Methods:
- Immunohistochemistry (IHC) was used to evaluate HIF-1α and VEGF-A protein expression.
- 34 de novo DLBCL tumor samples were analyzed.
- Correlation analysis was performed between marker expression and clinical/pathological characteristics.
Main Results:
- Overexpression of HIF-1α and VEGF-A (88.2%) was observed in the majority of DLBCL tumors.
- A significant positive correlation was found between HIF-1α and VEGF-A staining intensity (r=0.475; P=0.005).
- HIF-1α and VEGF-A expression correlated with serum lactate dehydrogenase (LDH) and tumor diameter.
Conclusions:
- Hypoxia, indicated by HIF-1α and VEGF-A overexpression, is prevalent in DLBCL tumors.
- These markers show potential as prognostic or pathogenic indicators in DLBCL.
- Further research is warranted to elucidate the specific role of hypoxia in DLBCL.
