Related Experiment Video

Updated: Sep 1, 2025

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.5K

Effects of miR-211-3p/RHBDD1 axis on cell proliferation, cell cycle progression, and epithelial-mesenchymal

Lei Chen1, Xiangyi Wang1, Yude Zhu1

  • 1Department of Neurosurgery, Inner Mongolia Baogang Hospital, Baotou City, Inner Mongolia Autonomous Region, P.R. China.

Folia Neuropathologica
|August 11, 2022
PubMed

Insights

MicroRNA-211-3p (miR-211-3p) directly targets RHBDD1 in glioma cells. Targeting this miR-211-3p/RHBDD1 interaction may offer a new therapeutic strategy for glioma treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Glioma is a primary brain tumor with limited treatment options.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • The specific role of miR-211-3p and its target RHBDD1 in glioma requires further elucidation.

Purpose of the Study:

  • To investigate the regulatory relationship between miR-211-3p and rhomboid domain containing 1 (RHBDD1) in glioma.
  • To determine the functional impact of the miR-211-3p/RHBDD1 axis on glioma cell behavior.
  • To explore the potential of targeting this axis for glioma therapy.

Main Methods:

  • Dual-luciferase reporter assay, RNA immunoprecipitation (RIP), RT-qPCR, and Western blot to confirm direct targeting.
  • Pearson's correlation analysis to assess expression correlation in glioma tissues.
  • Cell Counting Kit-8 (CCK-8), flow cytometry, and Transwell assays to evaluate cell proliferation, cell cycle, migration, and invasion.

Main Results:

  • miR-211-3p was confirmed to directly target RHBDD1 in glioma cells.
  • miR-211-3p expression was negatively correlated with RHBDD1 expression in glioma tissues.
  • RHBDD1 knockdown inhibited glioma cell proliferation, cell cycle progression, migration, and invasion, while miR-211-3p knockdown showed opposite effects.
  • RHBDD1 overexpression reversed the inhibitory effects of miR-211-3p on glioma cells, affecting key cell cycle and epithelial-mesenchymal transition markers.

Conclusions:

  • The miR-211-3p/RHBDD1 axis plays a significant role in regulating glioma cell proliferation, cell cycle, migration, and invasion.
  • Targeting the miR-211-3p/RHBDD1 pathway presents a promising novel therapeutic strategy for glioma treatment.
Keywords:
EMTRHBDD1miR-211-3pproliferationglioma

More Related Videos

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.7K
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

665

Related Concept Videos

The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.2K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.8K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K