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TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration
Binbin Li1, Liza Brusman1, Jacob Dahlka1
1Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO 80309, USA.
Development (Cambridge, England)
|August 11, 2022
Summary
Transmembrane protein 132A (TMEM132A) is crucial for mesoderm migration, which is essential for spinal neural tube closure in mammals. Disrupted mesoderm migration due to TMEM132A dysfunction may lead to birth defects like spina bifida.
Area of Science:
- Developmental biology
- Cell biology
- Genetics
Background:
- Coordinated mesoderm migration is vital for body plan organization during embryogenesis.
- The role of mesoderm migration in mammalian posterior neural tube closure remains largely unknown.
Purpose of the Study:
- To investigate the function of transmembrane protein 132A (TMEM132A) in mesoderm migration and posterior neural tube closure.
- To elucidate the molecular mechanisms by which TMEM132A influences cell migration and neural tube development.
Main Methods:
- Utilized Tmem132a-null mice and cell models to study TMEM132A function.
- Analyzed integrin regulation and downstream signaling pathways.
- Observed cell migration behaviors and neural tube closure in vivo and in vitro.
Main Results:
- TMEM132A is essential for both spinal neural tube closure and lateral migration of caudal paraxial mesoderm.
- TMEM132A regulates integrin activity and downstream signaling pathways controlling cell migration.
- Mesoderm migration, regulated by TMEM132A, is implicated in caudal neural fold elevation and successful neural tube closure.
Conclusions:
- TMEM132A plays a critical role in coordinating mesoderm migration necessary for mammalian spinal neural tube closure.
- Dysregulation of TMEM132A-dependent mesoderm migration may contribute to neural tube defects such as spina bifida.
Keywords:
Cytoskeletal remodelingIntegrin pathwayMesodermal cell migrationNeural tube closureSpina bifidaTransmembrane protein 132AMore Related Videos
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