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Gene profiling in dorso-ventral patterning of mouse tongue development
Tae-Young Kim1, Hyun-Geuk Jung1, Elina Pokharel1
1Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 2177 Dalgubeol-daero, Jung-gu, 41940, Daegu, Korea.
Genes & Genomics
|August 11, 2022
Summary
This study identified key signaling molecules in mouse tongue development using microarray analysis. Findings reveal distinct gene expression patterns in dorsal and ventral tongue tissues, aiding future research into tongue formation and diseases.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genomics
Background:
- The tongue, a complex oral organ, has distinct anatomical parts with intricate tissue organization.
- Understanding the molecular mechanisms driving tongue development is crucial for addressing related congenital anomalies and diseases.
Purpose of the Study:
- To identify signaling molecules involved in the development of the dorsal and ventral tongue parts.
- To utilize microarray analysis for a comprehensive gene expression profiling of tongue tissues.
Main Methods:
- Micro-dissection of dorsal and ventral tongue tissues from embryonic day 14 mice.
- RNA extraction and subsequent microarray analysis to profile gene expression.
- Validation of microarray data using quantitative reverse transcription polymerase chain reaction (qPCR) and whole-mount in situ hybridization.
Main Results:
- Microarray analysis identified 33,793 genes, with 931 equally expressed in both dorsal and ventral tongue parts.
- Differential gene expression revealed 725 genes upregulated in the ventral tongue and 1,672 in the dorsal tongue (fold-change > 1.5).
- Specific genes like Angiopoietin 2 (Angpt2), FGF18, Meox1, and Smoc2 were confirmed in the ventral tongue.
Conclusions:
- The identified signaling molecules provide a foundation for further investigation into tongue development.
- Future studies will employ loss- and gain-of-function mutations to elucidate the functional roles of candidate genes.
- This research offers valuable insights for understanding tongue morphogenesis and related pathologies.
