Related Experiment Video

Updated: Sep 1, 2025

A Robotic Platform for High-throughput Protoplast Isolation and Transformation
10:12

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

14.4K

Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis

Leonardo Soriano1, Ahmad A Omar2,3, Adriana Pinheiro Martinelli4

  • 1University of São Paulo, CENA, Piracicaba, SP, Brazil.

Methods in Molecular Biology (Clifton, N.J.)
|August 11, 2022
PubMed

Keywords:
BiotechnologyEmbryogenic callusIn vitro tissue culturePlant growth regulators

More Related Videos

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

25.2K
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana11:16

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana

Published on: August 17, 2009

36.4K

Related Concept Videos

Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
38.4K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.4K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K