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Somatic Embryogenesis in Citrus (Citrus spp.), var. Valencia
Joanne Moraes de Melo Souza1, Carolina Rossi de Oliveira2, Eveline Carla da Rocha Tavano2
1Federal Rural University of the Amazon, Belém, PA, Brazil.
Abstract:
Somatic embryogenesis has been obtained in many citrus cultivars; however, the efficiency of the system is genotype dependent and culture synchronization is important to reach more efficient systems. In this chapter we present a detailed protocol of somatic embryogenesis induction from nucellar tissue and the use of an alternative method of callus sieving for culture synchronization and embryo production. This is a simple method which can also be evaluated for other species aiming at better culture efficiency and somatic embryo production.
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