Use of Thin Cell Layer (TCL) to Obtain Somatic Embryogenesis

The thin cell layer (TCL) culture system was initially reported in relation to the model plant Nicotiana tabacum, giving rise to 47 years of continuous application and investigation on micropropagation and plant breeding of over 100 plant species or hybrids. The small sizes of the tissue sections (100 μm to 1-2 mm in thickness), its classification into transverse TCL (tTCL) or longitudinal TCL (lTCL) categories, and the interaction between the cultured cells and the culture medium are the main drivers of its efficacy in tens of plants for the induction of somatic embryogenesis, relative to the conventional in-vitro culture system. Furthermore, it promotes higher productivity and reduced time in the proliferation of cultures, which is key for the differentiation of cells and plant tissues. This chapter describes the main characteristics of the TCL sections, and the interaction between cells under in-vitro culture. In addition, it highlights the latest findings reporting the success of TCL in ornamental, herbaceous, woody, and recalcitrant plants. In most cases, studies on the use of TCL in combination with techniques such as bioreactors, histology, genetic transformation, and fidelity analysis, provide indisputable evidence that highlights the importance of this technique in plant biotechnology. Finally, the perspectives on TCL use are described, underlining the advantages and constraints of the technique for its continued use and future application.