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Gene network analysis of the transcriptome impact of methylated microRNAs on oral squamous cell carcinoma

Emilia Gabriela Avram1,2, Ioana Alexandra Moatar1,3, Viktorian Miok4

  • 1Doctoral School, Victor Babeș University of Medicine and Pharmacy, Timișoara, Romania.

Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University
|August 11, 2022
PubMed
Abstract

Insights

Epigenetic regulation by DNA methylation and microRNAs impacts oral cancer. This study identified four key microRNAs involved in oral squamous cell carcinoma (OSCC) development, offering insights into cancer pathology.

Area of Science:

  • Oncology
  • Epigenetics
  • Molecular Biology

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent head and neck cancer.
  • Epigenetic mechanisms, including microRNAs and DNA methylation, are crucial in regulating genes involved in cancer development.
  • Limited understanding of the genetic and epigenetic landscape in OSCC necessitates further investigation.

Purpose of the Study:

  • To investigate the transcriptomic effects of methylated microRNAs in OSCC.
  • To analyze the biological functions of these microRNAs using gene network analysis.
  • To elucidate the interplay between DNA methylation and microRNA expression in OSCC pathogenesis.

Main Methods:

  • Genome-wide DNA methylation profiling using the Infinium array on archived OSCC tissues.
  • Identification of differentially methylated microRNA loci after data quality control and normalization.
  • Literature-based analysis of OSCC transcriptomic data for microRNA target gene identification, followed by network and pathway enrichment analyses.

Main Results:

  • Analysis identified 1469 differentially hypomethylated loci in OSCC.
  • Four microRNAs of interest were identified: hsa-microRNA-124-3, hsa-microRNA-24-1, hsa-microRNA-769, and hsa-microRNA-4500.
  • Network and pathway analyses revealed multiple pathways modulated by DNA methylation-microRNA expression axes.

Conclusions:

  • The study characterizes the transcriptomic impact of four differentially methylated microRNAs in OSCC.
  • These findings contribute to understanding the role of epigenetic mechanisms in OSCC development.
  • This research enhances comprehension of how DNA methylation and microRNAs cooperate in OSCC pathology.
Keywords:
DNA methylationmicroRNAsnetwork analysisoral squamous cell carcinoma

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