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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Sep 1, 2025

A Microfluidic Technique to Probe Cell Deformability

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

Microfluidic deformability cytometry: A review.

Yao Chen¹, Kefan Guo¹, Lin Jiang¹

¹School of Mechanical Engineering, Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.

|August 11, 2022

Summary

This summary is machine-generated.

Accurate single-cell deformability measurement is crucial for biomedicine. Microfluidic technologies offer a promising solution for high-throughput analysis, advancing cell separation, disease diagnosis, and drug screening.

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Last Updated: Sep 1, 2025

A Microfluidic Technique to Probe Cell Deformability

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Published on: September 3, 2014

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Published on: March 13, 2017

Area of Science:

Biomedical Engineering
Cell Biology
Microfluidics

Background:

Cell deformability is vital for cellular functions like growth, differentiation, and cell cycle.
Single-cell deformability measurement is critical for applications in cell separation, disease diagnosis, and drug screening.
Current methods for measuring cell deformability require improvement in accuracy, robustness, and throughput.

Purpose of the Study:

To review recent advancements in microfluidic technologies for single-cell deformability analysis.
To categorize microfluidic approaches based on stress generation methods.
To discuss the advantages, disadvantages, and application scenarios of these technologies.

Main Methods:

Microfluidic technologies for cell deformability analysis.
Categorization based on stress generation: extrusion, hydrodynamic, electric stretching, optical stretching, and acoustic stretching.
Review of existing literature on microfluidic deformability cytometry.

Main Results:

Microfluidics provides a powerful platform for single-cell analysis, reducing complexity and reagent use.
Various microfluidic techniques (extrusion, hydrodynamic, electric, optical, acoustic) enable deformability measurements.
Each method presents unique advantages and disadvantages for specific applications.

Conclusions:

Microfluidic-based deformability cytometry is a rapidly advancing field with significant potential in biomedicine.
Future directions include enhancing detection performance, integrating diverse technologies, and expanding clinical applications.
These technologies are essential for improving cell-based diagnostics and therapeutics.