Photometric flow injection analysis of As(III) by using a homemade, LED-based flow-cell device and methyl orange...

Inorganic arsenic especially As(III) is considered a carcinogenic substance and its measurement is important in water samples. In this work, an inexpensive flow injection analysis system was designed for the photometric determination of As(III) at low concentrations. For this purpose, a light-emitting diode (LED) based photometer with a miniaturized detector, was fabricated and used as a determination apparatus and methyl orange was used as a detecting reagent. The fabricated photometer employed the LEDs, as a light source and the light detector. The λ_max of emission for emitter and detector LEDs were 525 and 625 nm, respectively. Determination of As (III) was based on its inhibition effect on the redox reaction between methyl orange and X₂ (Cl₂ or Br₂). The decolorization of the reaction products in the FIA system was monitored using the homemade flow cell detector. Analytical figures of merit including linear responses ranging from 0.03 to 3.0 mg/l of As(III) (r = 0.994), detection limits of 0.007 mg/l As(III), RSD% of 1.5% (n = 7), low reagent consumption per determination, and sampling throughput of 50 determinations per hour were achieved.