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Updated: Sep 1, 2025

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
Published on: December 29, 2013
Effect of electric current intensity on performance of polycaprolactone/FeS2-based mixotrophic biofilm-electrode
Xin Yang1, Zhiwei Tang1, Longqu Xiao1
1School of Civil Engineering and Architecture, Wuhan University of Technology, Wuhan 430070, China.
Abstract:
In this study, a bioelectrochemical system consisting of pyrite-based autotrophic denitrification (PAD) and heterotrophic denitrification (HD) was established to polish nitrate wastewater. The loading of electric current (EC) could stimulate the dissolution of pyrite. Appropriate EC (I ≤ 30 mA) was conducive to nitrate removal, too high EC (I = 40 mA) would inhibit nitrate removal and lead to an obvious accumulation of NO2--N and NH4+-N. Microbial analysis revealed that the increase of EC could inhibit the diversity of heterotrophic microbes, but appropriate EC (I = 10 mA) could increase the diversity of autotrophic microbes. The EC loading was conducive to the enrichment of iron autotrophic denitrifiers (Ferritrophicum), pyrite-oxidizing bacteria (Thiobacillus, Sulfurimonas), and sulfur autotrophic denitrifiers (Dechloromonas, Thiobacillus, and Arenimonas). The EC loading enlarged the contribution of PAD, making PAD a dominant pathway in denitrification.

