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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Sep 1, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Onepot-Seq: capturing single-cell transcriptomes simultaneously in a continuous medium via transient localization of

Dongju Shin1, Jungwon Choi1, Ji Hyun Lee2,3

  • 1Department of Chemistry, Yonsei University, Seoul, Korea.

Nucleic Acids Research
|August 11, 2022
PubMed
Summary

Onepot-Seq is a new device-free method for single-cell RNA sequencing. This technique captures high-quality single-cell transcriptomes in a continuous fluid, making cellular analysis more accessible.

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Last Updated: Sep 1, 2025

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Single-cell RNA sequencing (scRNA-seq) offers high-resolution analysis of cellular heterogeneity.
  • Existing scRNA-seq methods often require complex devices for cell compartmentalization, limiting accessibility.
  • There is a need for simplified, device-free methods for single-cell transcriptomic profiling.

Purpose of the Study:

  • To develop a device-free method for capturing single-cell transcriptomes.
  • To enable routine and accessible transcriptional profiling at single-cell resolution.
  • To demonstrate the utility of the method in complex biological samples and under chemical perturbations.

Main Methods:

  • Developed Onepot-Seq, a device-free method leveraging transient mRNA localization post-lysis.
  • Utilized a continuous fluid medium for simultaneous capture of single-cell transcriptomes.
  • Employed cell type-specific poly(A)-conjugated antibodies for targeted cell capture in complex populations.

Main Results:

  • Achieved high-quality single-cell profiles in mixed-species experiments.
  • Demonstrated effective capture of target cells within complex populations using antibody conjugation.
  • Successfully profiled chemical perturbations at single-cell resolution using Onepot-Seq.

Conclusions:

  • Onepot-Seq provides a simplified, device-free approach to single-cell RNA sequencing.
  • The method enhances accessibility for routine transcriptional profiling.
  • Onepot-Seq has the potential to accelerate discoveries in clinical and scientific research by enabling widespread single-cell analysis.