Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Centrosome-kinetochore interaction in multinucleate cells.

S Ghosh, N Paweletz

    Chromosoma
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Birth of the life sciences in The Netherlands and Belgium.

    Nature reviews. Molecular cell biology·2001
    Same author

    Walther Flemming: pioneer of mitosis research.

    Nature reviews. Molecular cell biology·2001
    Same author

    From Galen to Golgi: birth of the life sciences in Italy.

    Nature reviews. Molecular cell biology·2001
    Same author

    Lovastatin induces mitotic abnormalities in various cell lines.

    Cell biology international·1999
    Same author

    The influence of pneumoperitoneum used in laparoscopic surgery on an intraabdominal tumor growth.

    Cancer·1999
    Same author

    Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique.

    Cell biology international·1999

    Kinetochores, not centrosomes, terminate microtubule interactions in prematurely condensed chromosomes (PCCs). This explains abnormal mitotic behavior and micronuclei formation in PCCs during cell division.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Cytogenetics

    Background:

    • Centrosome-kinetochore interactions are crucial for accurate chromosome segregation during mitosis.
    • Prematurely condensed chromosomes (PCCs) arise in multinucleate cells and exhibit aberrant mitotic behavior.
    • The role of maturation promoting factor (MPF) in centrosome activation and kinetochore maturation is not fully understood.

    Purpose of the Study:

    • To investigate the interaction between centrosomes and kinetochores in PCCs.
    • To elucidate the mechanisms underlying abnormal mitotic behavior and nuclear re-formation in PCCs.
    • To determine the role of kinetochores in microtubule (MT) attachment and dynamics.

    Main Methods:

    • Induction of multinucleate cells using Colcemid treatment or cell fusion.

    Related Experiment Videos

  • Microscopic observation of prematurely condensed chromosomes (PCCs) and their interactions with centrosomes.
  • Analysis of kinetochore structure and microtubule attachment in various PCC groups.

    • Main Results:

    • PCCs, except for G2-phase PCCs, fail to develop their own spindle area, potentially due to MPF-mediated centrosome inactivation.
    • Kinetochore-centrosome interactions in PCCs are non-specific, showing variable chromosome-MT or chromosome-centrosome connections.
    • Kinetochore plates are present in all PCC groups, and their transformation to trilaminar kinetochores is independent of nuclear envelope breakdown or chromosome condensation, possibly MPF-induced.

    Conclusions:

    • Kinetochores, rather than centrosomes, act as termination sites for kinetochore microtubule assembly.
    • Non-specific kinetochore-centrosome interactions in PCCs explain observed abnormal mitotic behaviors.
    • MPF may orchestrate multiple mitotic events, including chromosome condensation, nuclear envelope breakdown, and kinetochore transformation.