Related Experiment Video
Updated: Sep 1, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
MicroRNA-375 inhibits laryngeal squamous cell carcinoma progression via targeting CST1
Feng Dai1, Zuojun Xie1, Qiming Yang1
1Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Pingxiang, China.
Objective:
This study aims to explore the effect and mechanism of miR-375 in Laryngeal Squamous Cell Carcinoma (LSCC) cell progression.
Methods:
LSCC cells (LSC-1 and TU177) were transfected with miR-375-mimic, miR-375-inhibitor or miR-375-mimic+oe-CST1. The expression of miR-375, CST1, MMP-2, and MMP-9 was measured. The effect of miR-375-mimic, miR-375-inhibitor or miR-375-mimic+oe-CST1 on cell biological functions, including cell proliferation, migration, invasion, and apoptosis, was also assessed. The potential relationship between CST1 and miR-375 was predicted by Jefferson software and validated by dual luciferase reporter gene assay.
Results:
Downregulated miR-375 expression was found in LSCC cells. Overexpression of miR-375 inhibited the viability and migration and promoted apoptosis of LSCC cells. Jefferson database and dual luciferase reporter gene assay confirmed that miR-375 directly targeted CST1. Overexpression of CST1 could reverse the anti-cancer effect of miR-375 overexpression in LSCC cells.
Conclusion:
Collected evidence showed that miR-375/CST1 axis was implicated in LSCC progression.
Level Of Evidence:
Level 3.
Related Concept Videos
01:22MicroRNAs
02:39lncRNA - Long Non-coding RNAs
02:23Abnormal Proliferation