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Updated: Sep 1, 2025
09:23
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
Published on: April 2, 2020
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Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer
Alexsandro Dos Santos1,2,3, Geneviève Ouellete1,2, Caroline Diorio2,4
1Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, Québec City, QC G1V 0A6, Canada.
Cancers
|August 12, 2022
Summary
Cytoskeleton-associated protein 2 (CKAP2) is overexpressed in invasive breast cancer (BC) and linked to poorer survival. CKAP2 knockdown impairs BC cell proliferation and migration, highlighting its role in tumorigenesis.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Mitotic dysregulation is a hallmark of cancer, leading to aneuploidy.
- Identifying key mitotic regulators is crucial for understanding cancer development.
Purpose of the Study:
- To identify novel mitotic regulators in breast cancer (BC).
- To investigate the role of cytoskeleton-associated protein 2 (CKAP2) in BC progression and its potential as a prognostic marker.
Main Methods:
- Analysis of a patient-derived BC gene-expression dataset.
- Human transcriptome analysis of breast lesions.
- CKAP2 gene knockdown in SKBR3 cells.
- Cell proliferation, migration, and 3D aggregate formation assays.
Main Results:
- CKAP2 is overexpressed in invasive BC compared to normal tissues and across all BC subtypes.
- Higher CKAP2 expression correlates with worse overall and relapse-free survival in ER-positive, HER2-negative BC patients.
- CKAP2 knockdown reduced cell proliferation, migration, and 3D aggregate formation in vitro.
Conclusions:
- CKAP2 is an important mitotic regulator in invasive breast cancer.
- CKAP2 shows potential utility as a prognostic marker for BC patients.
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