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Updated: Sep 1, 2025
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Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids
Published on: July 9, 2021
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Retinoic Acid Promotes the In Vitro Growth, Patterning and Improves the Cellular Composition of Human Pluripotent
Na Qu1, Braxton Jeffcoat1, Pritiprasanna Maity1
1Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA.
International Journal of Molecular Sciences
|August 12, 2022
Summary
All-trans retinoic acid (ATRA) improves human intestinal organoid (HIO) development. ATRA enhances HIO patterning, growth, and cellular composition, reducing neuronal contamination for regenerative medicine applications.
Area of Science:
- Stem cell biology
- Developmental biology
- Regenerative medicine
Background:
- Human intestinal organoids (HIOs) are derived from pluripotent stem cells.
- HIOs model human intestinal development and offer transplantation potential.
- Challenges include incomplete in vitro patterning and neuronal contamination.
Purpose of the Study:
- To investigate the effect of all-trans retinoic acid (ATRA) on HIOs.
- To improve in vitro growth, patterning, and cellular composition of HIOs.
Main Methods:
- Treatment of HIOs with all-trans retinoic acid (ATRA).
- Assessment of HIO growth, patterning, and cellular composition.
- Evaluation of neuronal contamination levels.
Main Results:
- ATRA treatment improved HIO patterning and increased organoid formation efficiency.
- ATRA enhanced epithelial growth and enriched intestinal subepithelial myofibroblasts (ISEMFs).
- Neuronal contamination in HIOs was reduced by ATRA treatment.
Conclusions:
- Manipulation of developmental signaling pathways, like ATRA, can optimize HIOs.
- ATRA improves HIO survival, patterning, and cellular makeup.
- Optimized HIOs show promise for regenerative medicine and disease modeling.
