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Related Experiment Video

Updated: Sep 1, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

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Distinct Hormone Signalling-Modulation Activities Characterize Two Maize Endosperm-Specific Type-A Response

Joaquín Royo1, Luís M Muñiz1, Elisa Gómez1

  • 1Departamento de Biomedicina y Biotecnología, Campus Universitario, Universidad de Alcalá, Alcalá de Henares, 28805 Madrid, Spain.

Plants (Basel, Switzerland)
|August 12, 2022
PubMed
Summary
This summary is machine-generated.

Maize response regulators ZmTCRR1 and 2, found in endosperm cells, modulate auxin and cytokinin signaling. These findings suggest a non-classical role in plant hormone perception within the inner endosperm.

Keywords:
maize endosperm transfer cellsresponse regulatorsignal transduction

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Type-A response regulators (ARRs) are key components of cytokinin signaling pathways.
  • In maize, ZmTCRR1 and ZmTCRR2 are type-A ARRs expressed in endosperm transfer cells (TCs) and regulated by ZmMRP1.
  • Unlike typical ARRs, ZmTCRR proteins accumulate in inner endosperm cells, suggesting a non-canonical function.

Purpose of the Study:

  • To investigate the function of maize response regulators ZmTCRR1 and ZmTCRR2.
  • To determine if ZmTCRR1 and ZmTCRR2 are involved in classical cytokinin signaling pathways.
  • To explore the role of ZmTCRR1 and ZmTCRR2 in modulating auxin and cytokinin perception.

Main Methods:

  • Ectopic expression of ZmTCRR1 and ZmTCRR2 in transgenic Arabidopsis thaliana.
  • Analysis of auxin and cytokinin signaling pathways in transgenic Arabidopsis.
  • Protein localization studies to determine ZmTCRR accumulation sites.

Main Results:

  • Ectopic expression of ZmTCRR1 modulated auxin signaling in Arabidopsis.
  • Ectopic expression of ZmTCRR2 significantly inhibited cytokinin perception in Arabidopsis.
  • ZmTCRR proteins were not detected in maize TCs but accumulated in inner endosperm cells.

Conclusions:

  • ZmTCRR1 and ZmTCRR2 do not function in classical, cell-autonomous cytokinin signaling.
  • These maize response regulators likely modulate cytokinin and auxin perception in inner endosperm cells.
  • The findings provide insights into non-canonical plant hormone signaling in maize endosperm development.