Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method for freezing synchronous mitotic and G1 cells.

M J Borrelli, M A Mackey, W C Dewey

    Experimental Cell Research
    |June 1, 1987
    PubMed
    Summary

    Researchers optimized a freezing protocol for cell cultures. This modified method preserved cell growth, division, and sensitivity after thawing, indicating successful cryopreservation of Chinese hamster ovary (CHO) cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Dodecafluoropentane emulsion delays and reduces MRI markers of infarction in a rat stroke model: a preliminary report.

    Magnetic resonance imaging·2014
    Same author

    Progress in dodecafluoropentane emulsion as a neuroprotective agent in a rabbit stroke model.

    Molecular neurobiology·2013
    Same author

    An open-source deconvolution software package for 3-D quantitative fluorescence microscopy imaging.

    Journal of microscopy·2009
    Same author

    THE SOL-GEL TRANSFORMATION IN GELATIN.

    The Journal of general physiology·2009
    Same author

    CELL SEGMENTATION USING HESSIAN-BASED DETECTION AND CONTOUR EVOLUTION WITH DIRECTIONAL DERIVATIVES.

    Proceedings. International Conference on Image Processing·2009
    Same author

    Arrhenius relationships from the molecule and cell to the clinic.

    International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·2009

    Area of Science:

    • Cell Biology
    • Cryobiology

    Background:

    • Cryopreservation is essential for maintaining cell lines.
    • Existing protocols may impact cell viability and function post-thaw.
    • Chinese hamster ovary (CHO) cells are widely used in research and bioproduction.

    Purpose of the Study:

    • To adapt and evaluate a modified freezing protocol for synchronous mitotic and G1 CHO cells.
    • To assess the post-thaw viability and functional integrity of cryopreserved CHO cells.

    Main Methods:

    • A modified cryopreservation protocol based on Kawamoto & Barrett (1986) was employed.
    • The protocol involved freezing cells in a solution with low sodium and high lactate/potassium concentrations.
    • Synchronous mitotic and G1 phase CHO cells were used for the study.

    Main Results:

    • Thawed CHO cells exhibited normal cell growth.
    • Cell division rates were unaffected by the cryopreservation process.
    • Plating efficiency remained high, comparable to unfrozen controls.
    • Hyperthermic sensitivity of the cells was preserved post-thaw.

    Conclusions:

    • The modified freezing protocol effectively cryopreserved synchronous CHO cells.
    • The protocol maintained critical cellular functions including growth, division, and sensitivity.
    • This method offers a promising approach for preserving cell line integrity in cryobiology.

    Related Experiment Videos