The Characteristics Analysis of a Microfluid-Based EGFET Biosensor with On-Chip Sensing Film for Lactic Acid...

Area of Science:

• Biosensor Technology
• Microfluidics
• Field-Effect Transistors

Background:

• Lactic acid (LA) detection is crucial in various fields, including healthcare and food science.
• Existing biosensors often face challenges with sensitivity, stability, and integration.

Purpose of the Study:

• To develop and characterize a microfluidic-based extended gate field-effect transistor (EGFET) biosensor for lactic acid detection.
• To integrate an on-chip sensing window (OCSW) and a microfluidic system for enhanced detection capabilities.
• To evaluate the suitability of the biosensor for wearable applications.

Main Methods:

• Fabrication of an EGFET biosensor featuring a six-metal-layer OCSW.
• Surface functionalization of the OCSW with ruthenium dioxide (RuO2) and lactase for specific LA binding.
• Integration of a microfluidic system for diversified LA detection.
• Packaging to ensure sealing and protection against environmental factors.
• Sensitivity analysis using a semiconductor parameter analyzer (SPA) for static and dynamic measurements.

Main Results:

• The fabricated EGFET biosensor demonstrated effective detection of lactic acid.
• Experimental results verified the sensing characteristics and performance of the biosensor.
• The biosensor exhibited stable operation and reliable measurements.

Conclusions:

• The developed microfluidic EGFET biosensor with an OCSW is a viable platform for lactic acid detection.
• The biosensor's low weight, low voltage operation, and simple manufacturing process make it suitable for wearable applications.
• This research contributes to the advancement of portable and efficient biosensing technologies.