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MiR-222 regulates the progression of oral squamous cell carcinoma by targeting CDKN1B

Qun Chen1, Wenjuan Wang2, Yali Wang1

  • 1Department of Endodontics, Hu'nan Xiangya Stomatological Hospital, Central South University Changsha 410000, Hu'nan Province, China.

American Journal of Translational Research
|August 12, 2022
PubMed
Abstract

Insights

MicroRNA 222 (miR-222) is elevated in oral squamous cell carcinoma (OSCC), promoting tumor growth by targeting CDKN1B. This study establishes a causal link between miR-222 and OSCC progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent malignancy.
  • MicroRNAs (miRNAs) play critical roles in cancer development.
  • The specific role of miR-222 in OSCC remains to be fully elucidated.

Purpose of the Study:

  • To investigate the causal relationship between microRNA 222 (miR-222) and oral squamous cell carcinoma (OSCC).
  • To explore the regulatory mechanism of miR-222 in OSCC progression.

Main Methods:

  • Cell viability was assessed using MTT assays.
  • Cell migration and apoptosis were measured by Transwell and flow cytometry.
  • The interaction between miR-222 and CDKN1B was confirmed via dual-luciferase reporter gene assays and Western blot.
  • Tumor growth was evaluated in vivo using xenograft models.

Main Results:

  • miR-222 levels were significantly increased in OSCC tissues and cells compared to normal controls.
  • Overexpression of miR-222 promoted OSCC cell proliferation, migration, and cell cycle progression while inhibiting apoptosis.
  • CDKN1B upregulation suppressed OSCC cell viability, migration, and invasiveness, and induced apoptosis.
  • miR-222 directly targets and inhibits CDKN1B, promoting tumor growth in vivo.

Conclusions:

  • MiR-222 is significantly upregulated in OSCC.
  • MiR-222 promotes OSCC progression by targeting and downregulating CDKN1B.
Keywords:
CDKN1BOral squamous cell carcinomamiR-222tumor progress

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