beta-D-mannosidase in human polymorphonuclear leukocytes and lymphocytes: a comparative study

All lymphocytes and polymorphonuclear leukocytes (PMNL) beta-D-mannosidase activities are adsorbed on DEAE-Trisacryl column at pH 7.0. Only one form is eluted with a 0.15 M linear gradient. The two enzymes isolated from either type of cells exhibit similar properties. The chromatographic profiles of beta-D-mannosidase from leukemic lymphocytes (chronic lymphoïd leukemia and hairy cells leukemia) differ from the normal ones by the presence of a more acidic minor form.