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beta-D-mannosidase in human polymorphonuclear leukocytes and lymphocytes: a comparative study.

B Colin, M Bernard, M J Foglietti

    The International Journal of Biochemistry
    |January 1, 1987
    PubMed
    Summary

    Beta-D-mannosidase enzymes from normal and leukemic lymphocytes share properties but differ chromatographically. Leukemic cells show an additional, more acidic enzyme form.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Cell Biology

    Background:

    • Beta-D-mannosidase is an enzyme found in various cell types, including lymphocytes.
    • Understanding enzyme properties can aid in disease diagnosis and characterization.

    Purpose of the Study:

    • To characterize beta-D-mannosidase from normal and leukemic lymphocytes.
    • To identify potential enzymatic differences between normal and cancerous lymphocytes.

    Main Methods:

    • Ion-exchange chromatography using DEAE-Trisacryl at pH 7.0.
    • Elution with a 0.15 M linear gradient.
    • Comparative analysis of enzyme properties and chromatographic profiles.

    Main Results:

    • Beta-D-mannosidase from lymphocytes and polymorphonuclear leukocytes (PMNL) adsorbs to DEAE-Trisacryl.

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  • A single enzyme form is eluted from normal cells.
  • Leukemic lymphocytes (chronic lymphoïd leukemia, hairy cells leukemia) exhibit an additional, more acidic beta-D-mannosidase form compared to normal lymphocytes.

    • Conclusions:

    • Beta-D-mannosidase enzymes from normal and leukemic lymphocytes share similar properties.
    • Chromatographic analysis reveals distinct enzymatic profiles, with leukemic cells possessing a unique acidic form.
    • This enzymatic difference may serve as a biomarker for specific leukemic conditions.