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beta-D-mannosidase in human polymorphonuclear leukocytes and lymphocytes: a comparative study
The International Journal of Biochemistry
|January 1, 1987
Summary
Beta-D-mannosidase enzymes from normal and leukemic lymphocytes share properties but differ chromatographically. Leukemic cells show an additional, more acidic enzyme form.
Area of Science:
- Biochemistry
- Enzymology
- Cell Biology
Background:
- Beta-D-mannosidase is an enzyme found in various cell types, including lymphocytes.
- Understanding enzyme properties can aid in disease diagnosis and characterization.
Purpose of the Study:
- To characterize beta-D-mannosidase from normal and leukemic lymphocytes.
- To identify potential enzymatic differences between normal and cancerous lymphocytes.
Main Methods:
- Ion-exchange chromatography using DEAE-Trisacryl at pH 7.0.
- Elution with a 0.15 M linear gradient.
- Comparative analysis of enzyme properties and chromatographic profiles.
Main Results:
- Beta-D-mannosidase from lymphocytes and polymorphonuclear leukocytes (PMNL) adsorbs to DEAE-Trisacryl.
- A single enzyme form is eluted from normal cells.
- Leukemic lymphocytes (chronic lymphoïd leukemia, hairy cells leukemia) exhibit an additional, more acidic beta-D-mannosidase form compared to normal lymphocytes.
Conclusions:
- Beta-D-mannosidase enzymes from normal and leukemic lymphocytes share similar properties.
- Chromatographic analysis reveals distinct enzymatic profiles, with leukemic cells possessing a unique acidic form.
- This enzymatic difference may serve as a biomarker for specific leukemic conditions.