Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

44.5K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.5K
DNA Isolation01:24

DNA Isolation

39.8K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct Spatial Organisation of Rho and RNA Polymerase in Salmonella Cells.

Molecular microbiology·2026
Same author

Rho-dependent transcription termination: mechanisms and roles in bacterial fitness and adaptation to environmental changes.

RNA (New York, N.Y.)·2025
Same author

Rho-dependent transcriptional switches regulate the bacterial response to cold shock.

Molecular cell·2024
Same author

Transcription-driven DNA supercoiling counteracts H-NS-mediated gene silencing in bacterial chromatin.

Nature communications·2024
Same author

Dynamics of macrophage polarization support <i>Salmonella</i> persistence in a whole living organism.

eLife·2024
Same author

Corrigendum: Scarless DNA Recombineering.

Cold Spring Harbor protocols·2023
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Sep 1, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.2K

Preparing Bacterial Genomic DNA.

Nara Figueroa-Bossi1, Roberto Balbontín2, Lionello Bossi3

  • 1Université Paris-Saclay, CEA, CNRS, Institut de Biologie Intégrative de la Cellule (I2BC), 91190 Gif-sur-Yvette, France.

Cold Spring Harbor Protocols
|August 12, 2022
PubMed
Summary
This summary is machine-generated.

We present two methods for bacterial chromosomal DNA purification: a fast silica membrane kit and a cost-effective CTAB-based method. Both yield high-quality DNA for downstream applications.

More Related Videos

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

19.1K
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

11.8K

Related Experiment Videos

Last Updated: Sep 1, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.2K
Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

19.1K
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

11.8K

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Bacterial chromosomal DNA purification is crucial for molecular biology techniques.
  • Existing methods vary in efficiency, cost, and suitability for different bacterial types.

Purpose of the Study:

  • To describe and compare two distinct protocols for bacterial chromosomal DNA isolation.
  • To evaluate their suitability for various downstream applications.

Main Methods:

  • Silica membrane technology utilizing selective DNA binding to a column in the presence of chaotropic salts.
  • Cetyl trimethyl ammonium bromide (CTAB) method for complexing polysaccharides and proteins, followed by chloroform-isoamyl alcohol extraction.

Main Results:

  • The silica membrane kit offers a quick, easy method yielding DNA suitable for most applications.
  • The CTAB method, while more cumbersome, is cost-effective and provides high DNA yields, particularly for Gram-negative bacteria.

Conclusions:

  • Both described methods effectively purify bacterial chromosomal DNA.
  • The choice of method depends on specific needs regarding speed, cost, and bacterial species.