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Luteolin restricts ASFV replication by regulating the NF-κB/STAT3/ATF6 signaling pathway

Yang Chen¹, Yanchen Guo², Zebu Song³

¹Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, PR China; African Swine Fever Regional Laboratory of China (Guangzhou), Guangzhou, PR China; Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, PR China.

Veterinary Microbiology

|August 12, 2022

Summary

Luteolin effectively inhibits African swine fever virus (ASFV) replication by targeting the NF-κB/STAT3/ATF6 pathway. This natural compound shows promise for developing new treatments against ASFV.

Area of Science:

Veterinary Virology
Immunology
Natural Product Chemistry

Background:

African swine fever (ASF) poses a significant economic threat to the global pig industry.
Luteolin, a flavonoid found in various plants, exhibits known antiviral properties.
Understanding luteolin's mechanism against ASF virus (ASFV) is crucial for therapeutic development.

Purpose of the Study:

To elucidate the antiviral mechanism of luteolin against ASFV.
To evaluate the efficacy of luteolin in inhibiting ASFV replication in porcine cells.
To investigate the impact of luteolin on key signaling pathways involved in ASFV infection.

Main Methods:

Porcine alveolar macrophages were treated with luteolin and infected with ASFV.
Assays included cell viability, hemadsorption, indirect immunofluorescence, Western blotting, and qRT-PCR.
Western blotting and qRT-PCR were used to analyze the expression of NF-κB, IL-6, STAT3, and ATF6.

Main Results:

Luteolin demonstrated dose-dependent inhibition of ASFV replication for up to 72 hours.
Luteolin interfered with multiple stages of the ASFV replication cycle.
Luteolin downregulated ASFV-induced expression of phosphorylated NF-κB, IL-6, and STAT3, and ATF6.

Conclusions:

Luteolin inhibits ASFV replication by modulating the NF-κB/STAT3/ATF6 signaling pathway.
The findings support luteolin as a potential candidate for developing novel anti-ASF therapies.
Further research into luteolin's therapeutic potential against ASFV is warranted.

Keywords:

African swine fever virus Luteolin NF-κB/STAT3/ATF6 signaling pathway Porcine alveolar macrophages

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