Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clec7a-targeted Res@GelMA hydrogels regulate macrophage polarization to reduce neuroinflammation and promote spinal cord repair.

Journal of orthopaedic surgery and research·2026
Same author

Reinstating Niche Failure in Diabetic Cranial Defects via Chronotaxic Signal-Amplifying Fluidic Biomimetic Hydrogel.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Spatiotemporal regulation of the bone immune microenvironment via a 'Zn<sup>2+</sup>-quercetin' hierarchical delivery system for bone regeneration.

Regenerative biomaterials·2025
Same author

<i>In situ</i> MgO nanoparticle-doped Janus electrospun dressing against bacterial invasion and immune imbalance for irregular wound healing.

Regenerative biomaterials·2024
Same author

The Porous SilMA Hydrogel Scaffolds Carrying Dual-Sensitive Paclitaxel Nanoparticles Promote Neuronal Differentiation for Spinal Cord Injury Repair.

Tissue engineering and regenerative medicine·2024
Same author

Effect of ubiquinol on electrophysiology during high-altitude acclimatization and de-acclimatization: A substudy of the Shigatse CARdiorespiratory fitness (SCARF) randomized clinical trial.

International journal of cardiology·2024

Related Experiment Video

Updated: Sep 1, 2025

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

37.1K

Graded-Three-Dimensional Cell-Encapsulating Hydrogel as a Potential Biologic Scaffold for Disc Tissue Engineering.

Zhixiang Li1,2, Yiwen Zhang1,3, Yupeng Zhao1

  • 1Department of Orthopedics, First Affiliated Hospital, School of Life Sciences, Bengbu Medical College, Bengbu, 233030, China.

Tissue Engineering and Regenerative Medicine
|August 13, 2022
PubMed
Summary

Researchers developed a novel tissue-engineered intervertebral disc (IVD) scaffold using gelatin methacrylate hydrogels. This scaffold supports cell survival and matrix expression, mimicking native IVD structure and function for potential lower back pain treatment.

Keywords:
Gelatin methacrylateHydrogelIntervertebral disk replacement

More Related Videos

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
12:37

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Published on: October 7, 2015

20.2K
Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.0K

Related Experiment Videos

Last Updated: Sep 1, 2025

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

37.1K
3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
12:37

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Published on: October 7, 2015

20.2K
Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.0K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Intervertebral disc (IVD) degeneration is a primary cause of lower back pain and disability.
  • Current strategies for IVD regeneration lack satisfactory methods to restore natural IVD properties.
  • Tissue engineering, particularly using scaffolds with cell cultures, shows promise for IVD regeneration.

Purpose of the Study:

  • To develop an integrated scaffold for intervertebral disc (IVD) replacement.
  • To evaluate the physical properties and biocompatibility of gelatin methacrylate (GelMA) hydrogels for IVD tissue engineering.
  • To simulate the native IVD's physiological structure and cellular microenvironment.

Main Methods:

  • Characterization of hydrogel physical properties using scanning electron microscopy and mechanical testing.
  • Seeding of nucleus pulposus (NP) cells and annulus fibrosus-derived stem cells (AFSCs) in GelMA hydrogels at varying concentrations.
  • Assessment of cell viability, adhesion, and extracellular matrix (ECM) expression (Type I collagen, Type II collagen, aggrecan).

Main Results:

  • GelMA hydrogels at different concentrations supported cell survival.
  • Hydrogel mechanical properties influenced cell adhesion and ECM component expression.
  • The developed scaffold mimicked the native IVD's structure, with distinct inner (NP) and outer (annulus fibrosus) regions.

Conclusions:

  • The integrated scaffold effectively simulates native IVD structure and function.
  • GelMA hydrogels provide a suitable microenvironment for IVD cells, influencing their behavior.
  • This research offers new approaches for creating functional tissue-engineered IVD replacements.