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EXPRESSION OF miRNA AS PROGNOSTIC MARKERS OF RENAL CELL CARCINOMA COURSE.
Yu Vitruk1, T Borikun2, O Rossylna3
1National Cancer Institute, Ministry of Health of Ukraine, Kyiv 03022, Ukraine. uvitruk@ukr.net
Experimental Oncology
|August 14, 2022
Summary
Aberrant microRNA (miRNA) expression, including miR-99b and miR-377, is linked to advanced renal cell carcinoma (RCC). A panel of miRNAs may serve as a prognostic marker for RCC progression.
Area of Science:
- Oncology
- Molecular Biology
- Biomarker Research
Background:
- Renal cell carcinoma (RCC) is a common adult solid tumor with a lack of reliable prognostic biomarkers.
- MicroRNA (miRNA) dysregulation is implicated in RCC progression, but expression patterns remain controversial.
Purpose of the Study:
- To investigate the expression and clinical significance of specific miRNAs (miR-99b, -144, -155, -210, -222, -302a, -377) in RCC.
- To determine if these miRNAs can serve as prognostic markers for RCC.
Main Methods:
- Retrospective analysis of miRNA expression in tumor tissue from 126 clear cell RCC patients.
- Quantitative polymerase chain reaction (qPCR) was used to measure miRNA levels.
Main Results:
- Overexpression of miR-99b, -222, -302a and low miR-155 levels correlated with advanced RCC stage.
- High levels of miR-99b and miR-377 were detected in patients with distant metastases.
- miRNA ratios (miR-99b, -144, -155, -210, -222, -302a) were associated with Fuhrman tumor grade.
Conclusions:
- Aberrant expression of the studied miRNAs is strongly associated with advanced RCC.
- A panel of these miRNAs shows potential as a useful prognostic marker for RCC course.
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