High-temperature Supercritical CO₂ Extraction of Lycopene from Tomato Powder for Enhancing Z-Isomerization and...

This study aimed to investigate the effect of extraction conditions (temperature, pressure, and entrainer content) on the total Z-isomer ratio and recovery of lycopene in the extracts obtained after supercritical CO₂ (SC-CO₂) extraction of lycopene from tomato powder, with a particular focus on high-temperature conditions (≥ 80°C). The results showed that high-temperature SC-CO₂ extraction promoted the thermal isomerization of lycopene in a temperature-dependent manner up to 120℃. For example, when lycopene extraction was carried out at 80, 100, 120, and 140°C and a pressure of 30 MPa with an entrainer, ethanol, for 180 min, the total Z-isomer ratios obtained were 25.0, 57.2, 67.2, and 67.0%, respectively. The entrainer content also affected the Z-isomer ratio of lycopene, but the pressure had little effect. Interestingly, when SC-CO₂ extraction was performed under high-temperature conditions (≥ 100°C), the extraction efficiency of lycopene was dramatically improved, e.g., when lycopene was extracted at 80, 100, 120, and 140°C under the same other conditions as above, the recovery rates of lycopene were 4.6, 28.5, 79.9, 84.8%, respectively. In general, SC-CO₂ extraction of fat-soluble components is performed at temperatures in the range of 40-80°C because the SC-CO₂ density decreases with increasing temperature, and thus, their solubility (extraction efficiency) decreases. However, our results showed that the lycopene recovery increased in a temperature-dependent manner, which might be due to the solubility enhancement associated with thermal Z-isomerization of lycopene (i.e., lycopene Z-isomers have greater solubility than the naturally occurring all-E-isomer). The high-temperature SC-CO₂ extraction of lycopene from tomato materials not only enhances the Z-isomer ratio of lycopene in the resulting extracts but also improves lycopene recovery. This new finding will greatly contribute to the value addition and cost reduction of natural lycopene sources obtained by SC-CO₂ extraction.