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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
Published on: October 21, 2022
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Comprehensive Analysis of circRNA-Associated-ceRNA Networks in Human Corneal Endothelial Dysfunction.
Ji-Ni Qiu1,2, Kun Shan1,2, Jun Xiang1,2
1Department of Ophthalmology, Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; and.
Cornea
|August 15, 2022
Summary
Circular RNAs (circRNAs) are dysregulated in corneal endothelial cells (CECs) under oxidative stress. Targeting circRNA-associated ceRNA networks may offer new therapies for corneal endothelial dysfunction.
Area of Science:
- Molecular Biology
- Genomics
- Ophthalmology
Background:
- Circular RNAs (circRNAs) are key regulators of gene expression via the competitive endogenous RNA (ceRNA) mechanism.
- circRNA-ceRNA networks are implicated in oxidative stress-related diseases.
- Corneal endothelial (CE) dysfunction due to oxidative stress is central to many corneal pathologies.
Purpose of the Study:
- To investigate circRNA-associated-ceRNA networks in oxidative stress-induced CE dysfunction.
- To identify molecular mechanisms underlying CE dysfunction.
Main Methods:
- Established an oxidative stress model using human corneal endothelial cells (HCECs) with H2O2 treatment.
- Performed high-throughput sequencing for circRNA, miRNA, and mRNA expression profiling.
- Utilized bioinformatics analyses (GO, KEGG) to identify dysregulated circRNAs and pathways.
Main Results:
- Identified 108 differentially expressed circRNAs (77 upregulated, 31 downregulated).
- GO analysis revealed enrichment in protein quality control and ubiquitin protein ligase binding.
- KEGG analysis highlighted the adherens junction pathway; potential therapeutic targets were identified.
Conclusions:
- circRNAs are significantly dysregulated in HCECs under oxidative stress.
- circRNA-ceRNA networks are closely linked to HCEC function.
- Targeting these networks presents a promising therapeutic strategy for CE dysfunction.
