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The synergistic effect of CDKN2B-AS1 and SPC25 on triple-negative breast cancer

Na Deng1,2, Keyan Chen3, Hua Fan2

  • 1Department of Breast Surgery, The First Hospital of China Medical University, Shenyang, China.

Annals of Translational Medicine
|August 15, 2022
PubMed
Abstract

Insights

Long non-coding RNA CDKN2B-AS1 and mRNA SPC25 promote triple-negative breast cancer (TNBC) progression. Their co-expression serves as a potential biomarker for TNBC diagnosis and prognosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNA (lncRNA) CDKN2B-AS1 and messenger RNA (mRNA) SPC25 are implicated in cancer development.
  • The synergistic role of CDKN2B-AS1 and SPC25 in triple-negative breast cancer (TNBC) remains underexplored.
  • Understanding this interaction may reveal novel therapeutic targets for TNBC.

Purpose of the Study:

  • To investigate the synergistic effect of CDKN2B-AS1 and SPC25 in TNBC.
  • To elucidate the underlying mechanism of TNBC progression driven by this axis.
  • To evaluate the potential of CDKN2B-AS1 and SPC25 as biomarkers for TNBC.

Main Methods:

  • Transcriptome analysis of TNBC using The Cancer Genome Atlas (TCGA) data.
  • Weighted gene co-expression network analysis to identify gene interactions.
  • In vitro validation including cell proliferation, migration assays, and qRT-PCR.

Main Results:

  • CDKN2B-AS1 was identified as carcinogenic and significantly upregulated in TNBC.
  • Elevated SPC25 expression was co-expressed with CDKN2B-AS1 and correlated with poor prognosis in TNBC patients.
  • Knockdown of CDKN2B-AS1 suppressed TNBC cell proliferation and migration.

Conclusions:

  • A novel cancer-promoting regulatory axis involving CDKN2B-AS1 and SPC25 was identified in TNBC.
  • The co-expression of CDKN2B-AS1 and SPC25 shows promise as a diagnostic and prognostic biomarker for TNBC.
  • This axis represents a potential therapeutic target for TNBC treatment.
Keywords:
Triple-negative breast cancer (TNBC)cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1)proliferationspindle component 25 (SPC25)

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