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Monoclonal antibody to human beta interferon: characterization and application
Hybridoma
|June 1, 1987
Summary
Seven monoclonal antibodies targeting human fibroblast interferon-beta (IFN-beta) were developed. These antibodies effectively neutralize IFN-beta's antiviral activity, aiding in its purification.
Area of Science:
- Immunology
- Virology
- Biochemistry
Background:
- Interferons (IFNs) are crucial cytokines with antiviral properties.
- Monoclonal antibodies (mAbs) offer high specificity for therapeutic and research applications.
- Human fibroblast interferon-beta (IFN-beta) plays a role in antiviral defense.
Purpose of the Study:
- To develop and characterize mouse hybridomas producing monoclonal antibodies against human fibroblast IFN-beta.
- To assess the neutralizing capacity of these mAbs against different IFN subtypes.
- To utilize mAbs for the purification of IFN-beta.
Main Methods:
- Isolation and characterization of seven stable mouse hybridomas secreting anti-IFN-beta mAbs.
- Testing neutralization activity against human fibroblast IFN-beta, IFN-alpha, and IFN-gamma.
- Purification of mAbs from hybridoma cultures.
- Affinity chromatography using anti-IFN-beta mAbs for protein purification.
Main Results:
- All seven hybridomas produced IgG1, kappa mAbs specific for human fibroblast IFN-beta.
- The mAbs neutralized the antiviral activity of fibroblast IFN-beta but not IFN-alpha or IFN-gamma.
- Two mAbs, YSB-1 and YSB-2, demonstrated high neutralization titers.
- Purified mAbs effectively neutralized IFN-beta in vitro.
- High purity human fibroblast IFN-beta and recombinant IFN-beta were obtained using mAb affinity chromatography.
Conclusions:
- Specific monoclonal antibodies against human fibroblast IFN-beta were successfully generated.
- These mAbs exhibit high specificity and neutralizing potency for IFN-beta.
- mAb-based affinity chromatography is an effective method for purifying IFN-beta.