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Updated: Sep 1, 2025

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Development of a Handheld Nano-centrifugal Device for Visual Virus Detection.

Zi-Rong Bi^1,2, Meng-Lu Hu², Yong-Zhong Jiang³

¹South China Normal University-Panyu Central Hospital Joint Laboratory of Basic and Translational Medical Research, Guangzhou Panyu Central Hospital, Guangzhou, 511400 China.

Journal of Analysis and Testing

|August 15, 2022

Summary

A novel handheld nano-centrifugal device improves gold nanoparticle assays for detecting low-concentration targets. This Hand-CRISPR platform offers accurate, field-deployable diagnostics for viruses like EBV and SARS-CoV-2.

Keywords:

CRISPR/Cas12a Centrifugal device Clinical diagnostics Gold nanoparticles Nucleic acids assay

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Last Updated: Sep 1, 2025

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Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

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Published on: June 23, 2022

Area of Science:

Nanotechnology
Biotechnology
Diagnostics

Background:

Gold nanoparticles (AuNPs) colorimetric assays are used in bioanalysis.
Current assays struggle with low-concentration targets due to faint color changes.

Purpose of the Study:

To develop an improved method for detecting low-concentration targets using AuNPs.
To create a field-deployable diagnostic platform for nucleic acid detection.

Main Methods:

Developed a handheld nano-centrifugal device to separate crosslinked and non-crosslinked AuNPs.
Integrated the device with the CRISPR/Cas12a nucleic acid recognition system.
Validated the platform for detecting Epstein-Barr virus (EBV) and SARS-CoV-2.

Main Results:

The nano-centrifugal device achieved over 6000 r/min within 10 s.
The Hand-CRISPR platform demonstrated high sensitivity and accuracy.
Achieved 100% consistency with reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) results for EBV and SARS-CoV-2 detection.

Conclusions:

The Hand-CRISPR platform shows great promise for point-of-care testing (POCT).
It can serve as a valuable supplement to standard nucleic acid testing in resource-limited settings.