Related Experiment Video
Updated: Sep 1, 2025
Murine Fecal Isolation and Microbiota Transplantation
Published on: May 26, 2023
Dataset of ileum bacterial diversity in mice after heart failure due to pressure overload
Martina E Spehlmann1,2, Dhiraj P Dhotre3, Nesrin Schmiedel1,2
1Department of Internal Medicine III, Cardiology, Angiology and Intensive Care Medicine, University Hospital of Schleswig-Holstein, Rosalind-Franklin Str. 12, Kiel 24105, Germany.
Abstract:
We recently reported the correlation of gut bacterial diversity with heart failure using a mouse model of heart failure due to pressure overload induced by transverse aortic constriction (TAC). We found that gut the bacterial diversity is significantly altered and is directly correlated to the severity of heart failure (Heart Failure Severity Closely Correlates with Intestinal Dysbiosis and Subsequent Metabolomic Alterations (Spehlmann, 2022). In addition, stool samples that were collected for the gut microbial diversity analysis, we dissected ileum from the mice after 42 days of TAC. The total DNA was extracted to identify the bacterial diversity resided in ileum using 16S rRNA gene amplicon shotgun sequencing and downstream bioinformatics analysis to determine if it is correlated to the heart failure.
