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Related Experiment Video

Updated: Sep 1, 2025

Ecosystem Fabrication EcoFAB Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

Ecosystem Fabrication EcoFAB Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

Published on: April 10, 2018

Tool and techniques study to plant microbiome current understanding and future needs: an overview.

Enespa¹, Prem Chandra²

¹Department of Plant Pathology, School of Agriculture, SMPDC, University of Lucknow, Lucknow, India.

Communicative & Integrative Biology

|August 15, 2022

Summary

Understanding microbial communities is crucial for environmental and human health. This review details essential tools and techniques for identifying and studying microbiomes and their ecological roles.

Keywords:

DNA fingerprinting Plant microbiome phytohormones polymerase chain reaction (PCR)transmission electron microscope (TEM)

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Last Updated: Sep 1, 2025

Ecosystem Fabrication EcoFAB Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

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Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

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Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

Area of Science:

Microbiology and Environmental Science

Background:

Microorganisms inhabit diverse environments, influencing human life, society, and ecosystems.
The plant microbiome, encompassing microbes in root, leaf, and internal plant tissues, plays critical roles in plant health and function.

Purpose of the Study:

To review the essential tools and techniques used for the isolation, purification, and identification of microorganisms.
To highlight the importance of these methods in understanding microbial communities and their ecological diversity.

Main Methods:

Microscopy for direct visualization.
Nucleic acid-based techniques like rRNA/rDNA sequencing.
Molecular fingerprinting and probing.
Clone libraries, DNA chips, and metagenomics for comprehensive community analysis.

Main Results:

Various tools enable rapid and accurate identification of microbial species.
Direct analysis and in situ applications are key benefits of modern techniques.
These methods are indispensable for elucidating the functions of microbiomes.

Conclusions:

Effective tools and techniques are fundamental for advancing our knowledge of microbiomes.
Understanding microbial roles is vital for environmental sustainability and human well-being.