Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

757
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
757
Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

894
Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
894
Electrodes: Overview01:17

Electrodes: Overview

1.8K
 Electrochemical measurements are conducted in an electrochemical cell composed of various components that control and measure the current and potential. One fundamental component is electrodes, conductive materials that enable electron transfer reactions at their surfaces.
There are two main types of electrodes in electrochemical cells. The first type, known as the working or indicator electrode, has a potential that is sensitive to the analyte's concentration and reacts to changes in...
1.8K
Potentiometry: Overview01:06

Potentiometry: Overview

2.6K
Potentiometry is an analytical technique that measures the potential difference between two electrodes in an electrochemical cell without drawing any significant current that could alter the solution's composition. This method employs an indicator electrode, which exchanges electrons with the analyte solution, and a reference electrode with a constant potential. Each electrode is immersed in a solution comprised of two half-cells. In a conventional setup, the reference electrode serves as...
2.6K
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

261
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
261
Standard Electrode Potentials03:02

Standard Electrode Potentials

44.9K
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
44.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Overcoming Limitations for Ultrasensitive <i>In Situ </i>pH Measurements in Marine Waters while Maintaining Traceability to Primary Standards.

Chimia·2026
Same author

Symmetrical pH Electrochemical Cell Coupled to Constant Potential Coulometry for Improved Sensitivity and Precision: Part 1. Fundamental Considerations.

ACS measurement science au·2026
Same author

Symmetrical pH Electrochemical Cell Coupled to Constant Potential Coulometry for Improved Sensitivity and Precision: Part 2. Submersible Probe for In Situ Measurements.

ACS measurement science au·2026
Same author

Zero-current chronopotentiometry for wired biosensors.

Mikrochimica acta·2025
Same author

Let Us Talk about pH, the Confusing Pillar of Aqueous Systems.

ACS sensors·2025
Same author

Spatially Resolved Ion Sensing by Voltammetric Ion Transfer Microscopy.

JACS Au·2025

Related Experiment Video

Updated: Sep 1, 2025

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
08:03

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Published on: April 18, 2013

17.4K

Unconditioned Symmetric Solid-Contact Electrodes for Potentiometric Sensing.

Polyxeni Damala1, Elena Zdrachek1, Tara Forrest1

  • 1Department of Inorganic and Analytical Chemistry, University of Geneva, Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland.

Analytical Chemistry
|August 15, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a symmetric potentiometric sensing setup that eliminates electrode conditioning, significantly reducing preparation time. This method enables stable, drift-free measurements for ions like nitrate and potassium using non-conditioned electrodes.

More Related Videos

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.5K
Author Spotlight: Microfluidic Channel-Based Soft Electrodes and Their Application in Capacitive Pressure Sensing
05:57

Author Spotlight: Microfluidic Channel-Based Soft Electrodes and Their Application in Capacitive Pressure Sensing

Published on: March 17, 2023

2.4K

Related Experiment Videos

Last Updated: Sep 1, 2025

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
08:03

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Published on: April 18, 2013

17.4K
Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.5K
Author Spotlight: Microfluidic Channel-Based Soft Electrodes and Their Application in Capacitive Pressure Sensing
05:57

Author Spotlight: Microfluidic Channel-Based Soft Electrodes and Their Application in Capacitive Pressure Sensing

Published on: March 17, 2023

2.4K

Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Sensing Technology

Background:

  • Electrode preparation in potentiometric sensing is time-consuming due to conditioning requirements.
  • Eliminating conditioning can speed up measurements but may affect membrane pre-equilibration.
  • Potential drift and instability are common challenges in potentiometric measurements.

Purpose of the Study:

  • To develop a symmetric potentiometric sensing setup to eliminate electrode conditioning.
  • To compensate for potential drift and improve measurement stability.
  • To enable rapid, accurate potentiometric detection of ions using non-conditioned electrodes.

Main Methods:

  • A symmetric setup was designed using identical indicator and reference electrodes in separate cells.
  • Potentials were measured between a reference cell and a sample cell containing identical electrodes.
  • The difference in potentials was used to eliminate instabilities not related to the sample.

Main Results:

  • The symmetric setup achieved Nernstian responses for nitrate and potassium detection without conditioning.
  • Potential drift was significantly reduced, with a standard deviation of 3 mV for E0 over 5 days (vs. 19 mV asymmetric).
  • Rapid stabilization was observed, with drift below 0.3 mV/min in under 6 minutes.
  • Successful application in river water demonstrated drift below 0.1 mV/min within 5 minutes.

Conclusions:

  • The proposed symmetric setup effectively eliminates the need for electrode conditioning in potentiometric sensing.
  • This approach significantly reduces measurement preparation time while maintaining high accuracy and stability.
  • The method offers a promising alternative for rapid and reliable ion detection in environmental samples.