Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Elephant prolactin: isolation and characterization.

C H Li, D Chung, T A Bewley

    International Journal of Peptide and Protein Research
    |April 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Purification and functional reconstitution of the cystic fibrosis transmembrane conductance regulator (CFTR).

    Cell·1992
    Same author

    Calcium-permeable channels in rat hepatoma cells are activated by extracellular nucleotides.

    The American journal of physiology·1991
    Same author

    Pharmacological characterization of the inhibitory activity of beta h-endorphin (beta h-EP), [Arg9,19,24,28,29]-beta h-EP, [Gln8,Gly31]-beta h-EP-Gly-Gly-NH2, in the neuroeffector junction of the mouse vas deferens.

    The Journal of pharmacy and pharmacology·1991
    Same author

    Insulin receptors prepared with iodoacetamide show enhanced autophosphorylation and receptor kinase activity.

    The Journal of biological chemistry·1991
    Same author

    [The study of pretreatment with diphenylhydantoin or D-tubocurarine on succinylcholine-induced adverse effects].

    Ma zui xue za zhi = Anaesthesiologica Sinica·1990
    Same author

    [Diagnostic significance of the cuticular patterns on leaf surface of Sarcandra glabra].

    Yao xue xue bao = Acta pharmaceutica Sinica·1990
    Same journal

    Structure-activity relationship of the ring portion in backbone-cyclic C-terminal hexapeptide analogs of substance P. NMR and molecular dynamics.

    International journal of peptide and protein research·1996
    Same journal

    Conformation of a water-soluble beta-sheet model peptide. A circular dichroism and Fourier-transform infrared spectroscopic study of double D-amino acid replacements.

    International journal of peptide and protein research·1996
    Same journal

    Application of the allyloxycarbonyl protecting group for the indole of Trp in solid-phase peptide synthesis.

    International journal of peptide and protein research·1996
    Same journal

    NMR solution structure of the receptor binding domain of Pseudomonas aeruginosa pilin strain P1. Identification of a beta-turn.

    International journal of peptide and protein research·1996
    Same journal

    Kinetics of inhibition of aminoacylase activity by dithiothreitol or 2-mercaptoethanol.

    International journal of peptide and protein research·1996
    Same journal

    Alanine scan and N-methyl amide derivatives of Ac-bombesin[7-14]. Development of a proposed binding conformation at the neuromedin B (NMB) and gastrin releasing peptide (GRP) receptors.

    International journal of peptide and protein research·1996
    See all related articles

    Elephant pituitary glands yielded prolactin, a hormone with 199 amino acids. This elephant prolactin showed differences in methionine content and lactogenic potency compared to ovine prolactin.

    Area of Science:

    • Endocrinology
    • Comparative Biochemistry
    • Protein Chemistry

    Background:

    • Prolactin is a key hormone regulating reproduction and metabolism.
    • Understanding prolactin structure and function across species provides insights into its evolution and diverse roles.

    Purpose of the Study:

    • To isolate and characterize prolactin from elephant pituitary glands.
    • To compare the structural and functional properties of elephant prolactin with those of other species, particularly ovine prolactin.

    Main Methods:

    • Isolation of prolactin from anterior pituitary lobes of elephants.
    • Amino acid sequencing of the NH2-terminal region.
    • Spectroscopic analysis (circular dichroism) to determine protein conformation.
    • Assessment of lactogenic potency.

    Related Experiment Videos

    Main Results:

    • Elephant prolactin comprises 199 amino acids with three disulfide bridges and two tryptophan residues.
    • The NH2-terminal sequence shows homology with ovine prolactin.
    • Elephant prolactin exhibits significantly lower methionine content and 18-34% of the lactogenic potency of ovine prolactin.
    • Circular dichroism spectroscopy indicates approximately 60% alpha-helical content, with spectral differences suggesting a unique tryptophan microenvironment.

    Conclusions:

    • Elephant prolactin shares structural similarities with other mammalian prolactins but possesses distinct biochemical properties.
    • Differences in methionine content and spectral characteristics may explain variations in lactogenic potency and protein conformation.