Chitosan and chitosan-derived nanoparticles modulate enhanced immune response in tomato against bacterial wilt
Konappa Narasimhamurthy1, Arakere C Udayashankar2, Savitha De Britto3
1Department of Microbiology and Biotechnology, Jnana Bharathi Campus, Bangalore University, Bangalore 560056, Karnataka, India.
Abstract:
The present study evaluated the priming efficacy of chitosan and chitosan-derived nanoparticles (CNPs) against bacterial wilt of tomato. In the current study, seed-treated CNPs plus pathogen-inoculated tomato seedlings recorded significant protection of 62 % against pathogen-induced wilt disease and subsequently better growth. The induced resistance was witnessed by a prominent increase in lignin, callose and H2O2 deposition, followed by superoxide radical accumulation in leaves. Additionally, chitosan and CNPs-treated tomato plants recorded a remarkable increase in the upregulation of phenylalanine ammonia-lyase (PAL), peroxidase (POX), polyphenol oxidase (PPO), catalase (CAT) and β-1, 3 glucanase (GLU) in comparison with untreated plants. The chitosan and CNPs-induced antioxidant enzymes were positively correlated with the stimulation of corresponding gene expression in CNPs treated plants related to pathogen-inoculated ones. The results of this study describe that how the application of chitosan and CNPs elicit defense responses at the cellular, biochemical and gene expression in tomato plants against bacterial wilt disease, thereby improve growth and yield.
