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Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
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Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Published on: March 29, 2010

A sensitive haemolytic assay of mouse complement

J H Berden, J F Hagemann, R A Koene

    Journal of Immunological Methods
    |January 1, 1978
    PubMed

    Under appropriate conditions the haemolytic activity of mouse complement can be measured in a 51Cr release assay. Modifications that increase the sensitivity are the following: (a) mouse 7 S anti-SRBC antibodies as amboceptor; (b) low amount of target cells; (c) ionic strength of 0.13 M NaCl in the test medium; (d) incubation temperature of 30 degrees C; (e) incubation time of 90 min; (f) total reaction volume of 1 ml.

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