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Related Experiment Videos

A sensitive haemolytic assay of mouse complement.

J H Berden, J F Hagemann, R A Koene

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

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    This study enhances the measurement of mouse complement haemolytic activity using a chromium-51 release assay. Optimized conditions significantly increase assay sensitivity for studying complement function.

    Area of Science:

    • Immunology
    • Complement System Analysis

    Background:

    • The haemolytic activity of mouse complement is crucial for immune responses.
    • Accurate measurement of complement activity is essential for immunological research.

    Purpose of the Study:

    • To optimize conditions for measuring mouse complement haemolytic activity.
    • To enhance the sensitivity of the chromium-51 release assay for mouse complement.

    Main Methods:

    • Utilized a chromium-51 (51Cr) release assay.
    • Investigated modifications including specific antibodies, cell concentrations, ionic strength, temperature, incubation time, and reaction volume.

    Main Results:

    • Identified optimal conditions for sensitive measurement of mouse complement haemolytic activity.

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  • Key factors include using mouse 7 S anti-SRBC antibodies, low target cell numbers, 0.13 M NaCl, 30°C incubation, 90 min incubation time, and a 1 ml reaction volume.

    • Conclusions:

    • The described modifications significantly enhance the sensitivity of the 51Cr release assay for mouse complement.
    • These optimized conditions provide a more robust method for studying mouse complement haemolytic activity.