A fluorescent immunoassay for the quantification of C-reactive protein

A sensitive fluorescent binding immunoassay (FBIA) was developed for measuring C-reactive protein (CRP), at a level of 20 ng/ml, in human and mouse sera. The advantages of the FBIA over radioimmunoassays are the long shelf-life of the FITC-labelled antibody, the re-use of the antibody bound microbeads, the short assay time and the use of inexpensive instruments.