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TBX3 activating PVT1 accelerates proliferation, migration, and invasion by modulating the miR-30a/LOX axis in

Jing Wen1,2, Wei Zhang3, Yan Zhou2

  • 1Department of Ultrasound Centre, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.

Endokrynologia Polska
|August 16, 2022
PubMed
Abstract

Insights

The transcription factor TBX3 promotes anaplastic thyroid carcinoma (ATC) progression by upregulating the long non-coding RNA PVT1. This pathway involves the miR-30a/LOX signaling axis, impacting cell proliferation, migration, and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Anaplastic thyroid carcinoma (ATC) is an aggressive cancer with poor prognosis.
  • Long non-coding RNAs (lncRNAs) are implicated in ATC development and progression.
  • Understanding the regulatory mechanisms of lncRNAs in ATC is crucial for therapeutic strategies.

Purpose of the Study:

  • To investigate the role and mechanism of PVT1 dysregulation in anaplastic thyroid carcinoma.
  • To elucidate the relationship between transcription factor TBX3 and PVT1 expression in ATC.
  • To explore the downstream signaling pathway regulated by TBX3 and PVT1.

Main Methods:

  • Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot were used to measure PVT1 and TBX3 levels.
  • Bioinformatic tools (JASPAR) and experimental assays (ChIP, luciferase) validated TBX3 binding to the PVT1 promoter.
  • Cellular assays (MTT, colony formation, transwell) assessed the impact of PVT1 on cell proliferation, migration, and invasion.

Main Results:

  • PVT1 expression was significantly upregulated in ATC tissues and correlated with TBX3 levels.
  • Downregulation of PVT1 suppressed proliferation, migration, and invasion of ATC cells.
  • TBX3 directly targeted the PVT1 promoter, enhancing PVT1 expression and modulating the PVT1/miR-30a/LOX axis.

Conclusions:

  • TBX3 acts as a positive regulator of ATC cell proliferation, migration, and invasion.
  • The TBX3-PVT1 axis plays a critical role in ATC pathogenesis.
  • Targeting the TBX3-PVT1-miR-30a/LOX pathway may offer a therapeutic strategy for ATC.
Keywords:
PVT1TBX3anaplastic thyroid carcinomainvasionmigrationproliferation

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