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ENDOCELL-Seud: a Delphi protocol to harmonise methods in endometrial cell culturing
Andrea Romano1, Sun-Wei Guo2, Jan Brosens3
1Department Obstetrics and Gynecology, GROW - School for Oncology and Reproduction, Maastricht University, Maastricht, The Netherlands.
Reproduction & Fertility
|August 16, 2022
Summary
Standardizing in vitro methods for culturing endometrial cells is crucial for reliable reproductive science research. This Delphi process aims to establish consensus on best practices for endometrial cell culture, ensuring reproducible results in the field.
Area of Science:
- Reproductive Biology
- Cell Biology
- In Vitro Models
Background:
- Endometrial cell culturing is vital for studying reproductive health and disease.
- Current lack of standardized protocols hinders reproducibility and reliability of research findings.
- Need for clear guidelines to improve in vitro research in reproductive science.
Purpose of the Study:
- To develop a consensus on standardized in vitro methodologies for culturing endometrial cells.
- To establish harmonized procedures for deriving, characterizing, and maintaining endometrial cells.
- To enhance the reliability and reproducibility of research using endometrial cell models.
Main Methods:
- Utilizing a Delphi process, an iterative consensus-building method.
- A steering committee will identify key areas for standardization.
- An expert panel (ENDOCELL-Seud Working Group) will participate in surveys to rate proposed methodologies.
Main Results:
- The Delphi process protocol is presented for establishing consensus on endometrial cell culture techniques.
- Ethical approval has been obtained for the study.
- Findings will be disseminated through publications and conferences.
Conclusions:
- Standardized in vitro methodologies are essential for advancing reproductive science.
- The ENDOCELL-Seud Project aims to provide crucial guidance for researchers.
- Consensus on best practices will improve the quality and comparability of research outcomes.
