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Updated: Sep 1, 2025

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Complete genome sequence of a novel lytic bacteriophage, PLG-II, specific for Lactococcus garvieae serotype II
Muhammad Akmal1,2, Issei Nishiki3, Nikita Zrelovs4
1Faculty of Agriculture, University of Miyazaki, Gauken Kibanadai Nishi 1-1, Miyazaki, 889-2192, Japan. nb21005@student.miyazaki-u.ac.jp.
Abstract:
A novel lytic siphophage, PLG-II, which is specific for Lactococcus garvieae serotype II strains that are pathogenic to fish, was isolated from seawater samples collected from Miyazaki Prefecture, Japan. Whole-genome sequencing showed that the PLG-II genome is a 32,271-bp double-stranded DNA molecule, with an average GC content of 37.74%. It contains 69 open reading frames (ORFs), 43 of which currently have no reliable functional annotation for their product, as well as a single tRNA. Comparative genomics analysis suggests that phage PLG-II might represent a novel species in the genus Uwajimavirus.
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