Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Beyond the Gold Electrode Standard: Using a Triazabutadiene-Maleimide Linker to Covalently Immobilize a Glycan-Polymer Biorecognition Element onto Carbon.

ACS applied bio materials·2026

Same author

Nanodisc-Forming Polymers for the Extraction of Membrane Proteins.

Biomacromolecules·2026

Same author

Core-block engineering enables control of ice recrystallisation inhibition in polymer nanoparticles.

Chemical science·2026

Same author

Antifreeze Glycolipid-Inspired Ice Recrystallization Inhibitors Based on End-Group Functionalized Poly(vinyl alcohol).

ACS macro letters·2026

Same author

Poly(sarcosine)-<i>block</i>-oligo(l-tryptophan) Copolymers as Noncovalent Inhibitors of Protein Aggregation.

ACS macro letters·2026

Same author

Cryopreservation of Avian Erythrocytes Using Macromolecular Cryoprotectants.

ACS polymers Au·2026

Search research articles

Related Experiment Video

Updated: Sep 1, 2025

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

Assay-ready Cryopreserved Cell Monolayers Enabled by Macromolecular Cryoprotectants.

Ruben M F Tomás¹, Akalabya Bissoyi¹, Thomas R Congdon²

¹Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, U.K.

Biomacromolecules

|August 16, 2022

Summary

Researchers developed a new cryoprotectant allowing cell monolayers to be frozen directly in multiwell plates. This innovation streamlines cell culture, making cells assay-ready within 24 hours post-thaw for drug discovery and research.

More Related Videos

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

Published on: June 15, 2014

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Published on: April 16, 2019

Related Experiment Videos

Last Updated: Sep 1, 2025

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

Published on: June 15, 2014

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Published on: April 16, 2019

Area of Science:

Cell Biology
Cryobiology
Biomedical Research

Background:

Cell monolayers are crucial for drug discovery and disease research.
Current cryopreservation methods require thawing cells into suspension, followed by extensive culturing before use.
This process is time-consuming and inefficient, delaying research and drug development.

Purpose of the Study:

To develop a novel cryopreservation method for intact cell monolayers in multiwell plates.
To enable rapid, assay-ready cell cultures post-thaw.
To streamline cell culture workflows for biomedical applications.

Main Methods:

Development and application of a synthetic macromolecular cryoprotectant.
Cryopreservation of cell monolayers directly within tissue culture multiwell plates.
Assessment of post-thaw cell recovery, morphology, viability, proliferation, metabolic activity, and response to toxicological challenge.

Main Results:

Successful cryopreservation of biomedically important cell monolayers in multiwell plates.
Achieved >80% cell recovery across three cell lines with low well-to-well variance.
Cryopreserved cells maintained morphology, membrane integrity, proliferative capacity, and metabolic activity, proving assay-ready within 24 hours.

Conclusions:

Synthetic macromolecular cryoprotectants can overcome limitations in current cell monolayer cryopreservation.
This method significantly reduces cell handling time, accelerating biomedical discovery.
The approach has the potential to revolutionize routine cell culture practices in research and drug development.