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Updated: Sep 1, 2025
08:59
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
Published on: September 27, 2019
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Dynamic Catalysis Guided by Nucleic Acid Networks and DNA Nanostructures
Yu Ouyang1, Pu Zhang1, Itamar Willner1
1The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Bioconjugate Chemistry
|August 16, 2022
Summary
This study introduces dynamic nucleic acid-enzyme and DNA nanostructures for controlled biocatalysis. These systems enable switchable catalytic cascades and model natural out-of-equilibrium transformations.
Area of Science:
- Biochemistry and Molecular Biology
- Nanotechnology
- Synthetic Biology
Background:
- Enzymes and DNAzymes are crucial catalysts.
- Controlling enzyme activity dynamically is challenging.
- Supramolecular assemblies offer platforms for complex functions.
Purpose of the Study:
- To develop novel nucleic acid-based systems for dynamic biocatalysis.
- To create switchable catalytic cascades using enzymes and DNA nanostructures.
- To model out-of-equilibrium biological processes.
Main Methods:
- Conjugating enzymes to nucleic acid networks.
- Engineering supramolecular DNA nanostructures with catalytic modules.
- Utilizing DNA origami for programmed enzyme positioning.
- Designing photo-switchable DNA nanomachines.
Main Results:
- Demonstrated dynamically switched biocatalytic cascades via triggered reconfiguration.
- Showcased feedback-driven dynamic biocatalysis by coupling networks.
- Developed dissipative, transient biocatalytic cascades.
- Engineered switchable DNAzymes and enzyme cascades using DNA nanostructures.
- Achieved photo-induced ON/OFF control of DNAzyme activity.
Conclusions:
- Nucleic acid-enzyme conjugates and DNA nanostructures are versatile platforms for dynamic catalysis.
- These systems provide models for natural out-of-equilibrium transformations.
- Future applications lie in advanced biocatalysis and synthetic biology.
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