Synthesis of cross-linked magnetic chitosan beads immobilised with bacteria for aerobic biodegrading...

Bacterial immobilisation is a technique by which bacteria are embedded into or adsorbed onto a carrier material thereby increasing bacterial tolerance to harsh environments. This technique can be used to enhance bacterial activity and to degrade pollutants. Immobilised bacterial beads that contain nanomagnetic particles allow bead recycling and reuse. In this study, our objective was to produce cross-linked nanomagnetic chitosan beads (MCBs) for the biodegradation of benzophenone-type UV filter chemicals such as 2,4-dihydroxybenzophenone (BP-1) and oxybenzone (BP-3). We found that the optimal concentration for creating these MCBs to be 1.2% by weight chitosan and 10% by weight nano-magnetite. We selected and isolated six benzophenone-n (BPs)-biodegrading bacteria identified to be various Pseudomonas spp., a Gordonia sp., and Rhodococcus zopfii; these were used to create MCBs that were able to effectively biodegrade BP-1 or BP-3 as a sole carbon source. Both BPs were effectively biodegraded and mineralised over 8 days in the presence of the selected MCB-immobilised bacterial strains. The highest pseudo-first-order constant rates for BP biodegradation were 8.7 × 10^-3 h^-1 for BP-1 (strain BP1-D) and 1.02 × 10^-3 h^-1 for BP-3 (strain BP3-1). The mechanical strength of the MCBs was measured to be above 90% based on recovered weight. The MCBs released their bacteria at rates in the range of 10⁴-10⁵ CFU/day. We also determined the pathway through which the BPs were being aerobically biodegraded based on the GC/MS profiles of the intermediates. Our findings provide a novel strategy for treating BPs via the use of reusable and recyclable MCBs that are cheap, easy and fast to synthesise.