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Temporary immersion systems (TISs): A comprehensive review.

Amir Hossein Mirzabe1, Ali Hajiahmad1, Ali Fadavi2

  • 1Department of Mechanics of Biosystem Engineering, Faculty of Engineering & Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj, Alborz, Iran.

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Temporary immersion systems (TISs) offer advantages for plant micropropagation and metabolite production over other bioreactors. This review details TIS structure, operation, and performance factors, suggesting future research directions.

Keywords:
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Area of Science:

  • Plant Biotechnology
  • Bioreactor Technology

Background:

  • Temporary immersion systems (TISs) are widely utilized in plant biotechnology.
  • TISs present distinct advantages over continuous liquid-phase bioreactors for micropropagation and secondary metabolite production.

Purpose of the Study:

  • To review the structure, operation modes, and configuration types of TISs.
  • To discuss the application of TISs in plant micropropagation and secondary metabolite production.
  • To analyze the advantages, disadvantages, and performance-affecting factors of TISs.

Main Methods:

  • Literature review of temporary immersion systems.
  • Analysis of TIS structure and operational principles.
  • Evaluation of TIS performance in plant micropropagation and secondary metabolite production.

Main Results:

  • TISs offer significant benefits for plant propagation and metabolite synthesis.
  • Key factors influencing TIS performance have been identified.
  • Comparison of TISs with other bioreactor types highlights their unique advantages.

Conclusions:

  • TISs are effective tools in plant biotechnology for specific applications.
  • Future research should explore advanced TIS designs, improved sterilization, and hybrid bioreactor systems.
  • Optimizing TIS design and integration can further enhance plant biotechnology outcomes.